Description

TB-500 (Thymosin Beta-4 Acetate Fragment)

TB-500 is a synthetic peptide modeled after the active region of Thymosin Beta-4 (Tβ4), a naturally occurring protein involved in tissue regeneration, cell migration, and angiogenesis. The active core of TB-500—Ac-SDKP (N-acetyl-seryl-aspartyl-lysyl-proline)—has been identified as the primary sequence responsible for Tβ4’s wound healing and regenerative effects.

In preclinical studies, TB-500 has shown the ability to promote actin upregulation, enhance cell migration, and support the remodeling of damaged tissue, particularly in the context of muscle, tendon, and cardiac injury. Its stability, water solubility, and systemic activity have made it a target of interest in angiogenesis, anti-inflammatory, and fibrosis research.

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Chemical Makeup

• Sequence (Active Region): Ac-Ser-Asp-Lys-Pro

• Molecular Formula: C212H350N56O78S

• Molecular Weight: ~4963.49 g/mol

• Form: Lyophilized powder

• Solubility: Water-soluble

• Stability: Stable at 2–8°C; store frozen for long-term use

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Research & Preclinical Investigations

Actin Binding and Cell Migration

TB-500 promotes the upregulation of G-actin, a monomer critical to cytoskeleton formation and cell motility. This mechanism is central to fibroblast migration, angiogenesis, and myogenesis—key components of tissue healing. Studies in animal models have shown accelerated recovery of damaged muscle fibers, enhanced collagen synthesis, and faster tendon regeneration.

Angiogenesis and Wound Healing

In dermal and vascular injury models, TB-500 has been shown to stimulate vascular endothelial growth factor (VEGF), increase capillary density, and improve blood flow to healing tissue. This suggests a potential role in ischemia research, surgical recovery, and chronic wound healing.

Cardiac and Neurological Studies

Rodent models of myocardial infarction and stroke have demonstrated TB-500’s potential to reduce fibrosis, improve vascularization, and enhance functional recovery. Additionally, TB-500 has shown promise in neuroregenerative studies, potentially by modulating inflammation and supporting axonal repair.

Anti-Fibrotic and Anti-Inflammatory Properties

TB-500 may modulate TGF-β1 and NF-κB pathways, helping reduce inflammation and fibrosis in liver, lung, and kidney models. These properties have made it a candidate for study in chronic inflammatory and fibrotic diseases.

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Research Use Only

TB-500 is intended strictly for in vitro and in vivo laboratory research. It is not approved for human use, therapeutic treatment, or diagnostic procedures. All use must comply with applicable institutional and laboratory safety standards.